Production of L-lactic acid from Cassava peel wastes using single and mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum

Production of L-lactic acid from Cassava peel wastes using single and mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum

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Production of L-lactic acid using cultures of Rhizopus oligosporus and Lactobacillus plantarum was investigated.Cassava peels were hydrolyzed by boiling for 1 Fat Burners h in either NaOH or HCl solutions followed by neutralization to a pH of 6.2.Reducing sugar produced from the hydrolysates increased with increasing concentrations of alkali or acid.

Samples hydrolyzed with HCl produced a maximum reducing sugar concentration of 402 mg/g substrate while alkali hydrolyzed samples produced a maximum reducing sugar concentration of 213 mg/g substrate.Hydrolysates were amended with 0.5% ammonium sulphate solution and inoculated with either single or Astragalus Root mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum and incubated for 48 h for lactic acid production.The best lactic acid production of 50.

2 g/100g substrate was observed in a mixed culture fermentation of acid hydrolyzed peels.Mixed culture fermentation of alkali hydrolyzed peels produced a maximum lactic acid concentration of 36.4 g/100g substrate.Un hydrolyzed cassava peels inoculated with a mixed culture of the microorganisms produced only 4.

6 g/100g substrate.This work reports an efficient use of cassava peels for bio-product formation through microbial fermentation.